FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16399275
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1079120.6580290897006118No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA176620.10769988307409932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG196550.036.1095923
AGACCGC197250.035.831574
GACCGCG200700.034.980155
TCAGACC214750.033.7773322
ACCGCGT211050.033.307046
CGCGTTC215750.032.6083158
CCGCGTT218450.032.1377647
CTCAGAC233300.031.9991361
GCGTTCT254800.027.7382769
CGTTCTC268950.026.79439510
TTCGACG11900.026.0279356
AATACGG270700.025.85143932
ATACGGA273450.025.63461533
TACGGAG271400.025.63257634
CAATACG282950.024.91991231
CCCAATA287800.024.71523529
ACTCCCC294800.024.347525
CCCCAAT293850.024.3156328
GAACGAT292050.024.28063845
ACGGAGA288300.024.2425735
Produced by FastQC (version 0.11.5)