FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25693547
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGCCGTCTTCTGCTTG272450.10603829825442163TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA41850.041.3853571
TATGCCG44250.038.3978448
CTCGTAT45250.037.2233244
CGTATGC47550.035.6709746
TCTCGTA48050.035.11561243
TCGTATG48900.034.9878145
ACGAATC50850.033.47218732
ATGCCGT50950.033.2326749
GTCACGA52150.033.0336829
CGAATCT52250.032.74469833
ATCGGAA53350.032.6239932
ACACGTC56150.030.89051413
ATCTCGT54800.030.84406942
CACGAAT56550.030.41131631
TCACGAA56350.030.2575130
CGTCTGA57900.029.90591816
AGTCACG60450.028.40044428
TGCCGTC60000.028.26924150
TCGGAAG62100.028.124473
GCCGTCT62600.027.14261451
Produced by FastQC (version 0.11.5)