FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22940566
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTGCTTG468710.20431492405200463TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA61000.048.4830131
TATGCCG66000.044.11231248
CTCGTAT67750.042.66792344
CGTATGC68500.042.37318846
TCTCGTA69350.041.81075343
TCGTATG71050.041.0182945
ATGCCGT71700.040.52319749
TGCCGTC72000.040.35434750
ATCGGAA74050.040.0103452
ATCTCGT73200.039.57139242
GCCGTCT73650.039.45019551
CGTCTGA75700.038.9659316
TCGGAAG77500.038.3426323
ACACGTC77050.038.24492313
CATCTCG76200.038.05217441
CGTCTTC78050.037.1589853
CCGTCTT79900.036.40120752
ACGTCTG81550.036.24304615
CGGAAGA82350.036.0118374
CACGTCT86600.034.09550514
Produced by FastQC (version 0.11.5)