FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26731979
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA34050.040.813671
TATGCCG39200.035.0669348
CTCGTAT40600.033.7850744
CGTATGC41200.033.4362546
TCTCGTA42100.032.65139443
TCGTATG43450.031.56901645
ATCGGAA45350.030.7033772
ATGCCGT47500.029.12575149
ACACGTC49050.029.04731413
ATCTCGT47650.028.9102542
TGCCGTC47950.028.60633750
CGTCTGA50850.027.7290416
TCGGAAG51600.027.0436533
GCCGTCT52150.026.64210551
CATCTCG55400.025.2386741
CGGAAGA56450.024.9290624
ACGTCTG58400.024.24522815
CGTCTTC56600.024.08361853
CCGTCTT57950.023.87378952
CACGTCT63600.022.30929614
Produced by FastQC (version 0.11.5)