FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20100603
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAC24650.045.71109433
GATCGGA24850.045.0743331
TATGCCG25200.044.8283648
GCTAACG26050.042.91475734
ACGAATC26550.042.21714438
CTCGTAT27300.041.7041544
CTAACGA27500.040.9737635
CGTATGC28150.040.340146
GTCACGC28100.040.30982229
ACGCTAA27850.040.24780332
TCTCGTA28200.040.16398243
CGAATCT28900.039.19124639
CACGCTA29850.037.65014331
ATGCCGT30000.037.55750749
ATCGGAA30600.037.1438872
TGCCGTC29950.037.12779650
TCGTATG30900.037.03632445
GCCGTCT30350.036.7356551
ACACGTC32050.035.43140813
TCGGAAG32200.035.105333
Produced by FastQC (version 0.11.5)