FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21292823
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC727770.3417912223287631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG145750.032.1212045
ACCGCGT147250.031.8748786
CCGCGTT148150.031.7997467
AGACCGC150250.031.3947814
CGCGTTC150750.031.3295678
CAGACCG151750.031.2205333
TCAGACC175250.028.3639262
TTCGACG18700.027.607696
CTCAGAC190300.027.5515441
GCGTTCT180050.026.2967669
AATACGG182200.026.01828632
TACGGAG181000.025.97903434
ATACGGA183650.025.81286233
GAACGAT196000.024.11153245
ACGGAGA197350.023.8865235
AACGATC200650.023.7292946
CGTTCTC208500.023.67065410
CAATACG205150.023.13639831
CCCAATA209800.022.6094929
CGGAGAG208400.022.56336436
Produced by FastQC (version 0.11.5)