FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20301133
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCCGTCTTCTGCTTG754200.3715063587830295TruSeq Adapter, Index 20 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA89000.052.068231
TATGCCG94750.047.8812348
CTCGTAT96250.047.0122144
CGTATGC97000.046.7705846
TCTCGTA97300.046.4440243
TCGTATG99000.045.9744845
ATGCCGT99200.045.67386249
ATCGGAA102850.045.217882
TGCCGTC102150.044.32596650
ATCTCGT102750.044.15281742
GCCGTCT103400.043.9041151
CGTCTGA104950.043.817516
ACACGTC105650.043.72262213
TCGGAAG107650.043.2837943
CCGTCTT107750.042.24115452
CGGAAGA110600.042.0472874
CGTCTTC108000.041.9869453
ACGTCTG111350.041.35200515
CACGTCT115150.040.19230714
AATCTCG116800.038.89213641
Produced by FastQC (version 0.11.5)