FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22270705
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTATGCCGTCTTCTGCTTG256590.11521413444253337TruSeq Adapter, Index 7 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38950.045.06013548
GATCGGA41000.043.4947971
CTCGTAT40850.043.18094344
TCTCGTA41700.042.1592843
CGTATGC41800.042.05842246
TCGTATG43050.041.0427745
ATGCCGT43700.040.09452449
ATCGGAA45400.039.437292
ATCTCGT46800.037.88014642
TGCCGTC46750.037.4782350
GCCGTCT47350.036.8788151
ACACGTC48700.036.5850513
TCGGAAG50550.035.5944983
CGTCTGA50000.035.5142516
CCGTCTT52700.033.30346352
CGTCTTC54150.032.7929953
CGGAAGA55000.032.7694634
ACGTCTG57250.031.06833515
CACGTCT61250.029.28030614
GTATGCC65400.027.24220347
Produced by FastQC (version 0.11.5)