FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24990627
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAATCTCGTATGCCGTCTTCTGCTTG4903821.9622636919033685TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA525250.058.0501861
CTCGTAT531450.057.5870844
TATGCCG533350.057.55994848
CGTATGC535600.057.40948546
TCTCGTA533100.057.3777143
ATCGGAA533900.057.3385162
TCGTATG538950.057.06905445
CGTCTGA538550.057.04366316
ACACGTC541800.057.0335813
TGCCGTC537900.057.02918650
TCGGAAG536100.056.9947243
ATGCCGT538900.056.93978549
ATCTCGT538050.056.86148542
GCCGTCT539500.056.8597151
CGGAAGA539750.056.5834774
ACGTCTG545200.056.37492815
CGTCTTC545950.056.16037453
CCGTCTT547250.056.10220752
CACGTCT553400.055.859414
AATCTCG551750.055.47122241
Produced by FastQC (version 0.11.5)