FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16520649
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC688260.4166059093683305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20000.034.9505586
GACCGCG142500.034.068945
ACCGCGT145400.033.490256
AGACCGC146950.033.2781034
CAGACCG147950.033.234813
CCGCGTT148550.032.8197027
CGCGTTC152050.032.083638
TCAGACC169850.030.0957782
TCGACGA24350.029.3123667
CGGTATA33550.028.8367320
CTCAGAC183200.028.8196771
GCGTTCT173200.028.2849169
GTTCGAC25250.028.0345465
AATACGG174350.027.94618632
TACGGAG173600.027.91401334
ATACGGA176200.027.65276533
CGACGAG25950.026.9367628
GAACGAT184200.026.4837245
CAATACG186450.026.3857531
CGTTCTC192850.026.21345710
Produced by FastQC (version 0.11.5)