FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24313169
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12750.018.74018948
GATCGGA13650.017.0804391
CGTATGC16000.015.85541446
TCTCGTA16500.015.19616843
CTCGTAT16050.015.07085544
ATGCCGT16600.014.92701549
TCGTATG18000.013.7659445
ATCGGAA19150.012.1744542
TGCCGTC20800.011.77108750
ATCTCGT22100.011.21207842
GCCGTCT24400.010.51795451
TCGGAAG24700.010.39173
ACACGTC24300.09.95420613
CGTCTGA24150.09.52748716
CATCTCG26600.09.09350641
CCGTCTT28600.08.97352552
CGGAAGA29750.08.9251294
CGTCTTC28600.08.66409353
ACGTCTG30150.08.12063215
CACGTCT33900.07.744429614
Produced by FastQC (version 0.11.5)