FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21272507
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTATGCCGTCTTCTGCTTG675570.31757892946045335TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG81950.051.75971248
CTCGTAT82400.051.69183744
TCGTATG83150.051.50938445
CGTATGC83800.050.6522546
TCTCGTA85000.050.11067243
GATCGGA87650.048.858641
ATGCCGT88700.047.8870249
ATCTCGT88900.047.87893742
ATCGGAA90150.047.7377932
ACACGTC93200.046.14564513
CGTCTGA95600.044.9238816
TGCCGTC97150.043.7212450
GCCGTCT102200.041.70515451
CATCTCG102300.041.66487541
TCGGAAG103500.041.637313
ACGTCTG103350.041.61221715
CCGTCTT104700.040.73855252
CGTCTTC106150.040.4877453
GTATGCC105150.040.42375647
CGGAAGA107400.040.401224
Produced by FastQC (version 0.11.5)