FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20404904
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCTATCATCTCGTATGCCGTCTTCTGCTTG3231691.5837810361666TruSeq Adapter, Index 23 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCTATCATCTCGTATGCCGTCTTCTGCTTGA288840.14155420677303848TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA377000.057.7281951
ATCGGAA420350.051.808532
TATGCCG413550.051.62058348
TCTCGTA415200.051.4651743
CGTATGC415700.051.43165246
CTCGTAT414550.051.424944
ACACGTC421200.051.3973613
CGTCTGA420250.051.3310516
TCGGAAG424500.051.2511523
ATCTCGT417100.051.2378142
TCGTATG418350.051.12701845
ATGCCGT417550.051.12607249
GCCGTCT419600.051.0028351
TGCCGTC419250.050.94690750
CGGAAGA427100.050.9448174
ACGTCTG428050.050.51973315
CATCTCG423550.050.4227141
CCGTCTT426000.050.28518752
CACGTCT431050.050.2570914
CGTCTTC426050.050.21697253
Produced by FastQC (version 0.11.5)