FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20153473
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGCCGTCTTCTGCTTG532010.26397931512846445TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG65650.053.37581348
GATCGGA66450.052.9577141
CTCGTAT68100.051.41222444
CGTATGC68300.051.3912346
ACGATAG68200.051.3494532
CGATAGA68150.051.1258633
TCTCGTA69300.050.6073543
ATCGGAA70200.050.3880842
GTCACGA70400.049.70287329
TCGTATG71400.049.28389745
TGCCGTC72550.048.42137550
ATGCCGT72450.048.36607449
CGTCTGA72900.048.35122316
CACGATA72450.048.33723431
GCCGTCT72750.048.1666351
ACACGTC73650.048.13883213
ATCTCGT73150.047.9844842
TCGGAAG73850.047.8947073
AGTCACG74000.047.6835928
TCACGAT74850.046.70850830
Produced by FastQC (version 0.11.5)