FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22516081
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGCCGTCTTCTGCTTG342930.15230447962947014TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42650.050.3474848
GATCGGA44950.048.1742861
ACGAATC45700.047.0573932
CTCGTAT46350.046.32837344
GTCACGA47250.045.57452429
CGAATCT47200.045.43682533
CGTATGC47650.045.25013446
TCTCGTA47850.044.81443443
ATCGGAA49050.044.337812
ATGCCGT48750.044.10809749
TCGTATG50300.043.15938645
TGCCGTC50150.042.99439650
ATCTCGT50950.042.37720542
TCGGAAG51650.042.1058963
ACACGTC52050.042.04831313
GCCGTCT51500.041.86735551
CGTCTGA53250.040.93456616
AGTCACG53350.040.58338528
TCACGAA53950.040.13480430
CACGAAT54000.039.93375831
Produced by FastQC (version 0.11.5)