FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22450164
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCTGCTTG363600.1619587277847948TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46400.049.015548
CTCGTAT47250.048.07130444
GATCGGA48500.046.90541
TCTCGTA48700.046.64002643
CGTATGC49300.046.25190446
TCGTATG49400.046.1582745
ATCGGAA50750.044.7585372
ATGCCGT51950.043.8357849
ACACGTC55250.041.27092413
CGTCTGA55900.041.1076516
ATCTCGT55250.041.0573642
TGCCGTC56400.040.32480650
GCCGTCT58150.039.06095551
TCGGAAG58750.038.7663423
CGGAAGA62000.036.9244844
ACGTCTG63900.036.0534815
CCGTCTT63750.035.6297252
CGTCTTC64450.035.25145353
CACGTCT68400.033.94029614
AATCTCG70100.032.65432441
Produced by FastQC (version 0.11.5)