FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19186419
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCTTG470660.24530893440823948TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58900.050.88606348
GATCGGA58750.050.592841
ACCGAAC59650.050.04908432
CTCGTAT59600.050.04092844
TCGTATG60200.049.7381945
CGTATGC60550.049.64556546
TCTCGTA60600.049.40988543
CCGAACT62700.047.66650433
ATCGGAA62700.047.657562
CGAACTT63450.047.1023334
ATGCCGT63800.047.0670549
ATCTCGT65600.045.59915542
ACACGTC66750.045.11950713
TCACCGA66400.044.96021330
CACCGAA66750.044.68096531
CGTCTGA68550.043.8069116
TGCCGTC68650.043.74184450
GTCACCG69100.043.1180629
GCCGTCT71150.042.28879551
TCGGAAG70700.042.0980153
Produced by FastQC (version 0.11.5)