FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30907383
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC733780.23741253020354391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC156200.032.0194934
CAGACCG157900.031.8616353
GACCGCG157800.031.578045
ACCGCGT160700.031.0766416
CCGCGTT164350.030.4039257
CGCGTTC167800.029.8669038
TCAGACC192700.027.2413392
CTCAGAC226900.024.5972791
TTCGACG23250.024.1059156
GCGTTCT214000.023.584419
ATACGGA210950.023.5760133
AATACGG214250.023.50299632
TACGGAG213250.023.18325634
GAACGAT231400.021.7728945
CAATACG235250.021.66829131
AACGATC237450.021.25540546
ACGGAGA239050.020.74274635
CCCAATA245150.020.6734229
CGTTCTC253850.020.50955810
AAGAACG249100.020.40319643
Produced by FastQC (version 0.11.5)