FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28175381
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1289570.4576938995075169No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA289910.10289479315293021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG261950.033.792583
AGACCGC261300.033.729844
GACCGCG270100.032.561375
ACCGCGT273600.032.24746
CCGCGTT278450.031.706297
CGCGTTC282400.031.2629158
TCAGACC292950.031.2041632
CTCAGAC317500.029.3810181
GCGTTCT323000.027.3880679
AATACGG322700.027.24154332
TACGGAG324350.026.983134
ATACGGA327200.026.91058333
CAATACG335800.026.37208231
TTCGACG20100.025.8292036
GAACGAT344300.025.56529445
CCCAATA352550.025.30369629
ACGGAGA348550.025.1096535
AACGATC354550.024.9260446
AAGAACG355300.024.89815543
CGTTCTC368450.024.78619410
Produced by FastQC (version 0.11.5)