FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27779609
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC677130.24375073097681108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38500.037.619566
CGACGAG43800.032.730338
TCGACGA44900.032.454057
GACCGCG151400.031.543855
ACCGCGT153300.031.3645026
GTTCGAC46250.031.1882385
AGACCGC158600.030.3355274
CCGCGTT159050.030.2117357
CGCGTTC161100.029.754058
CAGACCG164450.029.3642353
CGGTATA73450.028.35308820
ACGAGGA71550.025.643110
TCAGACC205050.025.5497742
GCGTTCT196000.024.6366949
CTCAGAC227000.024.400061
AGTTCGA61000.024.3725784
AACGACG33500.024.12639416
AATACGG200100.023.78126532
TACGGAG200450.023.46010434
ATGACGG84600.023.36098516
Produced by FastQC (version 0.11.5)