FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24474171
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTATGCCGTCTTCTGCTTG632850.25857872775343443TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG76200.052.03153248
GATCGGA76300.051.701251
CGTATGC78800.050.23988346
CTCGTAT78900.049.91448644
TCTCGTA79400.049.488743
TCGTATG80100.049.46133445
ATGCCGT81550.048.5053849
ATCGGAA82600.047.817362
ACACGTC83800.047.27342213
ATCTCGT84350.046.79482342
TGCCGTC85850.046.2820350
CGTCTGA85600.046.03926516
GCCGTCT88200.045.0848251
TCGGAAG88200.044.9491123
CATCTCG90650.043.379641
CGGAAGA91700.043.2013244
CGTCTTC92800.042.881853
CCGTCTT93050.042.6714852
ACGTCTG92700.042.60853215
CACGTCT98600.040.32719814
Produced by FastQC (version 0.11.5)