FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33898480
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1376710.4061273543828514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC287800.032.5964854
GACCGCG288350.032.4422345
ACCGCGT290000.032.34926
CAGACCG293850.032.03583
CCGCGTT298600.031.4158427
CGCGTTC300750.031.1814488
TCAGACC337900.028.8382512
ATACGGA339450.027.84467733
TACGGAG336950.027.82363734
AATACGG343200.027.56621732
CTCAGAC372300.027.2547421
GCGTTCT348450.027.1076749
GAACGAT355350.026.77327245
CAATACG359450.026.49227531
AACGATC366750.026.0617146
ACGGAGA365350.025.72539935
AAGAACG368950.025.71425843
AGAACGA374650.025.41752644
ACGATCA382250.025.0353947
CCCAATA384400.025.02527229
Produced by FastQC (version 0.11.5)