FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23626145
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC672260.2845407069160034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG151500.032.8239025
AGACCGC153000.032.810614
CAGACCG155000.032.5803683
ACCGCGT156400.031.870166
CGCGTTC157000.031.6731518
CCGCGTT160200.031.0957137
TTCGACG26700.030.1568286
TCAGACC180600.029.3830832
CTCAGAC202750.027.5384311
AATACGG193600.026.21856132
ATACGGA196000.025.8976333
TACGGAG193850.025.89577934
GTTCGAC31700.025.493925
TCGACGA33200.025.1409517
GCGTTCT202450.024.6790819
CAATACG208400.024.62559731
CGGTATA41300.024.21093220
GAACGAT214800.023.6995445
CGTTCTC220450.023.6674310
ACGGAGA213500.023.59528235
Produced by FastQC (version 0.11.5)