FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22252990
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCCGTCTTCTGCTTG448800.2016807628997272TruSeq Adapter, Index 20 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54500.051.5231748
CGTATGC55800.050.42852846
GATCGGA56850.049.4738461
CTCGTAT57150.049.28891444
TCTCGTA57300.049.0569343
ATGCCGT59050.047.50318549
TGCCGTC59550.047.05480650
TCGTATG60500.046.85221545
ATCTCGT60300.046.7630442
GCCGTCT61000.046.0813551
ATCGGAA61800.045.694942
ACACGTC62500.045.40014313
TCGGAAG63150.044.4377253
CGTCTGA64400.043.69438616
CGTCTTC66600.042.33950853
CCGTCTT66800.041.99196652
CGGAAGA68800.041.0028534
ACGTCTG69800.040.56756215
CACGTCT72250.039.35521314
AATCTCG71850.039.2047541
Produced by FastQC (version 0.11.5)