FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23570801
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC324300.13758548129102613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA105350.025.50894752
CCTAAGC106450.025.27306454
CCCTAAG110900.024.20575153
CTAAGCT123550.021.65575255
AGCCCTA138650.021.29708751
TAAGCTC129300.020.87545256
GTTCCTA51250.020.20328315
CCCGTAT22000.019.9839361
TTCAACG145000.018.96075813
CCCGGAT59200.018.8402881
CAACGTT191850.018.65124115
CCGGCTT95500.018.3497262
CGTTGTG192550.018.30759418
CTCGGTT60900.018.1690161
CGAGGCG49850.018.16694654
ACGTTGT199150.017.9674917
AAGGGAC197250.017.88628627
AACGCTA62900.017.82139623
GCTCCCC168500.017.70738259
AACGTTG200150.017.70093216
Produced by FastQC (version 0.11.5)