FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481811
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTATGCCGTCTTCTGCTTG396340.21444868146308824TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47450.052.89890348
GATCGGA49650.050.9468081
CTCGTAT49850.050.3526644
CGTATGC50950.049.55447846
TCTCGTA51150.049.18825543
ATGCCGT51650.048.76866549
ATCGGAA52550.048.3693772
TCGTATG52850.047.88457545
GCCGTCT52600.047.3831951
TGCCGTC53100.046.8259350
ACACGTC55600.045.622913
TCGGAAG55650.045.399873
ATCTCGT55800.045.30067442
CGTCTGA56800.044.7622916
CGGAAGA60000.042.3543364
CGTCTTC59150.042.18605853
CCGTCTT59450.041.92356552
ACGTCTG62350.040.82569515
CACGTCT62400.040.7929814
AATCTCG64700.039.2059741
Produced by FastQC (version 0.11.5)