FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11668718
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC337260.28902918041210696No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT132110.11321723603227021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG11000.030.0359046
AGACCGC79750.028.9626984
GACCGCG79650.028.9249885
CAGACCG80600.028.693863
ACCGCGT81800.028.2734076
CGCGTTC84550.027.318578
CCGCGTT86700.026.641127
GTTCGAC12400.025.6927175
TCAGACC95700.025.0604252
CGGTATA15700.023.2983120
CTCAGAC118050.022.1760061
GCGTTCT106200.021.7771649
CTACACG47350.021.55579857
TCGACGA15950.020.7141517
CGTTCTC124450.019.2473510
AATACGG117700.019.17386632
TACGGAG116950.019.12033734
ATACGGA118550.018.96173733
TACACGT54550.018.65659158
CAATACG123200.018.58128431
Produced by FastQC (version 0.11.5)