FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23767253
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC328690.1382953259259705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA100500.025.888952
CCTAAGC106200.024.99937254
CCCTAAG106400.024.70285653
AACGCTA36050.023.56657623
GTTCCTA56250.023.07487915
CTAAGCT120250.022.1275155
ACGCTAC39800.021.49434524
AGCCCTA132900.021.1308851
CGCTACC41100.020.88624825
TAAGCTC128900.020.64283456
CTACACG42100.020.39043857
ACTATCG18250.020.20522758
CAACGTT180600.019.33940915
GTCCGTT20850.019.1049421
CCCGTAT19500.019.0657521
TTCAACG137550.019.06551713
CGTTGTG186350.018.64769718
AACGAAT43500.018.58090839
GCCCTAT48700.018.5354753
ACGAATT44150.018.50779540
Produced by FastQC (version 0.11.5)