FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31080077
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1516100.48780445428111385No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA380200.12232916926171065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG321500.032.4075053
AGACCGC321400.032.36254
GACCGCG322150.032.0718465
ACCGCGT328000.031.5577686
CCGCGTT338500.030.5696667
TCAGACC355400.030.3128972
CGCGTTC342800.030.1948138
CTCAGAC383300.028.851731
TTCGACG28250.027.1488656
GCGTTCT388500.026.7492359
CGTTCTC431200.024.8391810
AATACGG422050.024.60288232
ATACGGA425500.024.36098133
TACGGAG423100.024.32479134
CAATACG444850.023.60052331
GAACGAT448050.023.23351545
ACGGAGA448050.022.98337635
AACGATC456400.022.91836246
CCCAATA461050.022.8994929
CCTCTCA469250.022.78780719
Produced by FastQC (version 0.11.5)