FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24201797
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTATGCCGTCTTCTGCTTG248357210.261932202802958TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2620000.058.835821
ATCGGAA2634000.058.5813262
TCGGAAG2642200.058.4980553
ACACGTC2643600.058.44420213
CGGAAGA2645850.058.38394
CGTCTGA2644200.058.36548216
CTCGTAT2636650.058.28351644
TCTCGTA2637350.058.2626943
TATGCCG2642300.058.25501648
ACGTCTG2651100.058.23658415
CGTATGC2646150.058.2237746
TCGTATG2647750.058.2030745
CACGTCT2654850.058.18432214
ACCCGTG2630800.058.18317432
CACCCGT2633050.058.1697931
ATCTCGT2641600.058.14215542
ATGCCGT2647450.058.14169349
TCACCCG2639000.058.1414830
GCACACG2658150.058.11985811
TGCCGTC2648400.058.11304550
Produced by FastQC (version 0.11.5)