FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27426507
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTATGCCGTCTTCTGCTTG1463240.5335130718614659TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA163600.055.769751
TATGCCG173000.052.15838248
CGTATGC174000.051.9589246
CTCGTAT172650.051.92099444
TCTCGTA173350.051.6432743
ATCGGAA177850.051.38222
TGCCGTC177300.051.01004850
TCGTATG177900.050.88617345
ATGCCGT179300.050.40815449
TCGGAAG181650.050.3305363
ACACGTC180900.050.28779213
GCCGTCT180700.050.0665851
ATCTCGT179050.050.0486442
CGTCTGA182400.049.7439616
CGGAAGA188900.048.242494
CGTCTTC187550.048.0810453
CCGTCTT188950.047.80284552
ACGTCTG190950.047.60930615
CACGTCT194450.046.8282114
AATCTCG193100.046.4529241
Produced by FastQC (version 0.11.5)