FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26763513
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT370800.1385468342664881No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC363110.1356735193918676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA64050.030.30516623
ACGCTAC65200.029.81588624
CGCTACC67250.028.77540425
CTACACG69500.027.97152757
AAAACGC76550.025.70354321
TACACGT77800.024.75991658
GAAAACG80950.024.19706720
GCCCTAA113350.023.00601852
CCTAAGC115500.022.7565154
ACACGTT85250.022.4583359
AAACGCT88850.022.07885622
CCCTAAG122900.021.45835153
GCTCTAC93700.020.62108854
GCCGGCT106850.020.5998211
CCGGCTT115900.020.5156382
TCACGCT100800.020.3390258
TTTCGGT96950.020.05130447
CTAAGCT131400.020.00286755
GTCCGTT26100.019.8962541
TTCACGC103850.019.7132027
Produced by FastQC (version 0.11.5)