FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22030482
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC570720.2590592434609465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC127700.030.5471744
GACCGCG127100.030.4547945
ACCGCGT128700.030.231646
CAGACCG130950.029.879173
CCGCGTT134850.028.8742377
CGCGTTC136050.028.5111528
TCAGACC158350.025.7712572
AATACGG169800.023.34887132
CTCAGAC185650.023.1756781
ATACGGA171600.023.08597633
TACGGAG170450.023.08587834
GCGTTCT171850.022.8634769
CAATACG182450.021.85934631
GAACGAT182300.021.58528145
ACGGAGA185350.021.27778235
AACGATC186650.021.25606246
CGTTCTC199550.020.39928210
CCCAATA198350.020.39010229
AAGAACG195000.020.28531343
AGAACGA197100.020.08414844
Produced by FastQC (version 0.11.5)