FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26022975
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTTCTAATCTCGTATGCCGTCTTCTGCTTG881500.33873913340038947TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG104200.053.1676748
CTCGTAT105450.052.39754544
GATCGGA106100.052.0049551
TCTCGTA106150.051.96864343
CGTATGC107000.051.88664646
TCGTATG107950.051.51200545
ATCGGAA110400.050.2847252
ATCTCGT111550.049.6121542
ATGCCGT112300.049.3551649
ACACGTC113650.048.95057313
CGTCTGA114400.048.39815516
GTCACGT116600.047.5879129
TGCCGTC117450.047.16589450
AGTCACG118600.046.78541628
TCGGAAG119050.046.5072253
CGTGTTC119600.046.00808733
ACGTCTG120800.045.8584315
GCCGTCT121200.045.80540551
ACGTGTT122350.045.28077732
CGGAAGA123500.044.807574
Produced by FastQC (version 0.11.5)