FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23370992
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23850.039.1849861
TATGCCG25450.036.04643248
CTCGTAT27400.033.26484744
CGTATGC27950.032.6102646
TCGTATG29200.031.31528945
TCTCGTA29100.031.22017543
ATCGGAA30450.031.008292
TGCCGTC30350.030.3241350
ATGCCGT30850.029.92827249
GCCGTCT31750.028.89409451
ATCTCGT33050.027.84586342
ACACGTC34800.027.37876513
CGTCTGA34150.027.20805216
TCGGAAG35050.026.682183
CATCTCG36400.025.60727141
CGTCTTC37350.024.72008353
CGGAAGA38200.024.6363564
CCGTCTT39750.023.22754952
ACGTCTG40750.023.0908915
CACGTCT45100.021.2561414
Produced by FastQC (version 0.11.5)