FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27348208
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1660500.6071695812756726No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA360290.1317417214319856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG369250.034.1706543
AGACCGC369250.034.0827674
GACCGCG369700.033.873715
ACCGCGT373250.033.6384776
CCGCGTT380050.033.0582587
CGCGTTC382900.032.7968528
TCAGACC401800.032.5262372
CTCAGAC418450.032.046231
GCGTTCT425700.029.672699
TTCGACG31150.028.7905566
CGTTCTC468900.027.79478610
CCCAATA440600.025.86385729
CCTCTCA451250.025.56732419
CCCCAAT453050.025.23775728
AATACGG450200.024.7103732
ACTCCCC463800.024.6146325
TACGGAG450500.024.58913834
ATACGGA452950.024.54080433
CACTCCC472800.024.40188824
TCGACGA37350.024.2471587
Produced by FastQC (version 0.11.5)