FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38235039
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1179130.3083899038261737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC254900.032.787884
GACCGCG255300.032.5747345
CAGACCG258250.032.5453343
ACCGCGT257950.032.274396
CCGCGTT280250.029.7255637
CGCGTTC287200.029.0575898
TTCGACG31150.028.6959886
TCAGACC306550.028.3613782
CTCAGAC353650.025.7707541
GCGTTCT346250.024.2384729
GTTCGAC39750.022.3391175
TCGACGA40550.021.9698527
CGGTATA52300.021.82840520
CGTTCTC399700.021.5581410
TACGGAG390950.020.29814134
AATACGG412200.020.22504232
CGACGAG43150.020.1675078
ATACGGA396150.020.13595633
CAATACG442650.019.12699331
GAACGAT442250.018.89775545
Produced by FastQC (version 0.11.5)