FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25400131
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTATGCCGTCTTCTGCTTG543370.21392409354109237TruSeq Adapter, Index 9 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67900.051.04980548
CTCGTAT69000.050.32147244
GATCGGA68650.050.3138921
CGTATGC70300.049.43288446
TCGTATG70550.049.13226745
TCTCGTA71150.048.8423343
TATCTCG71800.048.15326741
ATCGGAA73400.047.3408362
ATGCCGT74750.046.17015549
ATCTCGT75400.045.97262642
ACACGTC77450.044.6367813
TGCCGTC78300.044.11454450
CGTCTGA80150.043.06068816
GCCGTCT81500.042.42046751
TCGGAAG81400.042.3985183
CGTCTTC83900.041.31249253
CGGAAGA83950.041.145794
CCGTCTT85300.040.5998652
ACGTCTG89550.038.60651815
CACGTCT95400.036.29998414
Produced by FastQC (version 0.11.5)