FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18322931
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC399150.21784178524713102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG14400.027.8603446
AGACCGC111200.027.7241524
CAGACCG113750.027.2841953
GACCGCG111800.027.126035
ACCGCGT114100.026.655276
CGCGTTC118400.025.5874848
CCGCGTT119350.025.408537
TCAGACC138950.023.843392
CTCAGAC158350.022.4836941
GCGTTCT141950.021.5288189
CGGTATA25150.021.46438220
GTTCGAC18800.020.8703025
CGTTCTC161750.020.13372410
TCGACGA21250.019.1570667
CGACGAG20000.018.4369418
CTACACG50700.017.1058557
AATACGG181350.016.91732832
TACGGAG178000.016.85398934
CCGTACG33650.016.8330544
ATACGGA184600.016.66688333
Produced by FastQC (version 0.11.5)