FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19109944
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT227320.11895377610735018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA56450.022.94075252
CGAGCTA17000.021.51673911
CCTAAGC60900.021.50664754
AACGCTA31550.021.50469423
ACGCTAC32250.021.49527424
GTTCCTA38500.020.68760115
CCCGTAT16650.020.5587311
CCCTAAG64050.020.49500353
GTTTACG21250.019.8508726
CGCTACC34900.019.4404925
AGCCCTA74950.018.7738751
TACGAGC19650.018.614999
TTACGAG20050.018.5378238
GTCCGTT18600.018.244731
CTAAGCT72400.018.2125855
CCTACTC44050.017.81318918
CTACACG37450.017.80160357
GTCCGGT20600.017.189631
AGTTACC45900.017.1593634
AAAACGC41000.017.12375621
Produced by FastQC (version 0.11.5)