FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20986466
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17200.034.81496448
GATCGGA17750.033.2552951
CTCGTAT20200.029.9364944
CGTATGC20150.029.57159246
TCTCGTA21600.027.9961643
TCGTATG21650.027.5227545
ATCGGAA22100.027.1086622
ATGCCGT23850.025.84986749
ATCTCGT25350.023.97108342
TGCCGTC26600.023.06652550
CGTCTGA26750.023.04750616
ACACGTC26650.022.91244713
GCCGTCT28300.021.36819551
TCGGAAG29150.020.7479763
CATCTCG30700.019.88979541
CGGAAGA30700.019.5080094
CGTCTTC31650.019.29324753
CCGTCTT32150.018.80968752
ACGTCTG34150.018.13956615
CACGTCT38100.016.25895714
Produced by FastQC (version 0.11.5)