FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20789711
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTCTTCTGCTTG504090.24247090303467903TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59800.052.4316448
GATCGGA61200.051.41791
CTCGTAT61950.050.374444
CGTATGC62600.050.27492546
TCTCGTA62650.050.04696743
TGCCGTC63600.049.02066850
ATGCCGT65250.048.1879149
TCGTATG65500.048.0490145
ATCGGAA65750.047.9021452
CGTCTGA66500.047.6370716
ACACGTC66950.047.53750613
GCCGTCT66200.047.22905351
ATCTCGT66550.047.15840542
TCGGAAG69100.045.6551553
CGTCTTC69650.045.01668553
CCGTCTT71050.043.963652
CGGAAGA72050.043.7858624
ACGTCTG72600.043.7568115
CACGTCT75500.042.07608414
AATCTCG76600.041.16370841
Produced by FastQC (version 0.11.5)