FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20800368
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1023690.49214994657786826No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA210170.10104148157378753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG215250.034.1393975
CAGACCG218050.034.134763
AGACCGC218900.033.934754
CGCGTTC224700.032.7809838
ACCGCGT225650.032.656286
TCAGACC241300.032.3742
CCGCGTT229800.032.066467
CTCAGAC262150.030.874341
TTCGACG21350.030.2590896
AATACGG259900.028.16058232
ATACGGA264200.027.7794133
TACGGAG260150.027.610934
GCGTTCT273000.027.0237149
CAATACG275800.027.01837231
CGTTCTC292650.026.30807510
GAACGAT283850.026.1888945
ACGGAGA276000.026.04652635
CCCAATA294350.025.80680829
AACGATC290950.025.78346
CCTCTCA304250.025.43212119
Produced by FastQC (version 0.11.5)