FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10904690
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC341340.3130212780005667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC81700.033.328114
CAGACCG82450.032.9891623
GACCGCG82050.032.5747155
CGCGTTC83300.032.1908158
ACCGCGT83350.032.1728326
CCGCGTT83950.032.011857
TTCGACG11050.029.9010726
TCAGACC96600.029.8065472
CTCAGAC107100.028.134461
GCGTTCT102350.026.37229
CGTTCTC116200.024.47326910
GTTCGAC13750.024.244145
TCGACGA14400.023.1488327
AATACGG109100.022.90233232
CGGTATA16550.022.81524320
TACGGAG107950.022.70907634
ATACGGA110550.022.6553133
CCCAATA119000.021.88904829
CCTCTCA121600.021.83343119
CAATACG117800.021.56149931
Produced by FastQC (version 0.11.5)