FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21335627
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTATGCCGTCTTCTGCTTG14907076.986937857509414TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1598100.058.7611471
ATCGGAA1608200.058.5242922
TCGGAAG1609800.058.410933
ACACGTC1613400.058.36469713
CGTCTGA1612700.058.29857616
CGGAAGA1612650.058.256494
TATGCCG1611050.058.24845548
CTCGTAT1605300.058.20588344
TCTCGTA1606100.058.16710343
CGTATGC1615050.058.1569446
ACGTCTG1619450.058.09929715
ATGCCGT1615900.058.0695249
TCGTATG1618300.058.06822245
CACGTCT1621800.058.0587614
TGCCGTC1616500.058.04978650
ATCTCGT1609200.058.02755442
GCCGTCT1617700.057.99213451
CGTCTTC1619150.057.91930853
GCACACG1626500.057.91819811
CCGTCTT1622750.057.82872452
Produced by FastQC (version 0.11.5)