FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23610356
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16550.028.1756671
TATGCCG16800.028.09380548
CGTATGC19100.024.55628646
TCGTATG20550.023.39778345
TCTCGTA20350.023.19287143
CTCGTAT20850.022.77816644
ATCGGAA21750.021.3032262
ATGCCGT22100.021.223349
ATCTCGT25800.018.75094242
ACACGTC25500.018.2774613
TGCCGTC26600.017.85469250
GCCGTCT26300.017.72186951
CGTCTGA27550.017.1316116
TCGGAAG29900.015.6882513
CATCTCG30850.015.49026241
CCGTCTT32500.014.8861252
CGTCTTC32650.014.27561853
CGGAAGA32750.014.1427014
ACGTCTG35000.013.56927815
CACGTCT36350.013.55223714
Produced by FastQC (version 0.11.5)