FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19738573
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTATGCCGTCTTCTGCTTG197740.10017948105975037TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28100.046.3962348
GATCGGA29000.045.2733041
ACCGACT29650.044.07954432
CTCGTAT29950.043.23489444
TCTCGTA30800.042.2336943
CGTATGC30800.041.85019346
CCGACTG31650.041.47885533
TCGTATG31300.041.46436745
CACCGAC31600.041.35944431
ATCGGAA31950.041.0916672
CGACTGG32500.039.9401634
ATGCCGT32550.039.60028549
GCCGTCT33550.038.77161851
TCGGAAG34250.038.673753
TGCCGTC33650.038.65639550
GTCACCG34050.038.38331629
ACACGTC35050.037.86964413
TCACCGA36050.036.7448930
ATCTCGT35850.036.11998742
CGTCTGA36900.035.5716216
Produced by FastQC (version 0.11.5)