FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17166524
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC319680.18622290686221626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC76700.031.2312434
CGCGTTC77250.030.9316088
GACCGCG77250.030.8179445
CAGACCG78800.030.6610013
CCGCGTT81000.029.4995927
ACCGCGT80950.029.4461296
TCAGACC99300.025.3115632
AATACGG99250.023.59967232
CTCAGAC116900.023.5092281
ATACGGA99950.023.43439133
TTCGACG14750.023.4006296
TACGGAG97400.023.3210334
CAATACG106200.022.33302331
GAACGAT109500.021.41794645
GTTCGAC16150.021.1891775
AACGATC113600.020.69687746
CCCAATA116550.020.60264829
GCGTTCT118350.020.3892389
ACGGAGA114050.020.14921635
AAGAACG118950.019.84039743
Produced by FastQC (version 0.11.5)