FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20727893
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTATGCCGTCTTCTGCTTG542710.2618259366738337TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62100.052.863948
GATCGGA64500.051.6802981
CGTATGC64700.050.83071546
CTCGTAT64550.050.81175244
TCTCGTA65100.050.38246543
TCGTATG66750.049.40218745
TGCCGTC66550.048.8858450
ATGCCGT67550.048.72978249
GCCGTCT67050.048.5652851
ATCGGAA68950.048.5516932
ACACGTC69100.047.8930513
ATCTCGT70250.046.85689542
CGTCTGA71050.046.4123316
TCGGAAG72050.046.3692473
CGTCTTC72800.044.93200353
CGGAAGA75200.044.191444
ACGTCTG74950.044.1155515
CCGTCTT74200.043.84572252
CACGTCT78600.042.40466314
AATCTCG81150.040.63580741
Produced by FastQC (version 0.11.5)