FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25742466
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC373760.14519199520356751No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT295430.11476367493308529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA117600.025.81172252
GTTCCTA69550.025.40638415
CCTAAGC123500.024.65026954
CCCTAAG129100.023.60385953
CTAAGCT136850.022.28869655
AGTTACC79250.021.99853534
TAAGCTC144250.021.41134856
GCCCTAT62100.020.71113253
AACGCTA39100.020.5211623
CCTACTC85950.020.3868218
AGCCCTA161200.020.33080551
GTCCGTT25250.019.748411
ACGCTAC40950.019.66611324
AACGAAT56350.019.00282939
CCCGTAT22750.018.9355641
GCACTAA64300.018.90163658
TTCAACG165900.018.83053813
CGCTACC42800.018.8160625
ACATGCC97550.018.7791086
ACGAATT56500.018.74353640
Produced by FastQC (version 0.11.5)